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Research Group of Pharmacokinetics & Protein-ligand Interactions

Anthony Tsarbopoulos, Associate Professor

 

Research Associates 

  • Dr. Alexios Leandros Skaltsounis, Professor (Department of Pharmacy, National and Kapodistrian University of Athens)
  • Dr. Evangelos Gikas, Assistant Professor (Department of Pharmacy, National and KapodistrianUniversity of Athens)
  • Dr. Fotini Lamari, Associate Professor (Department of Pharmacy, University of Patras)
  • Evangelia Karkoula, MSc Pharmacist, PhD candidate
  • IoannaValentiniDagla, MScPharmacist, PhD candidate
  • Dr. Dimitris  Anagnostopoulos, Chemist
  • Dr. Nikolaos  Koulakiotis, Chemist

 

 

History and Achievements

Dr. Tsarbopoulos group has a long experience in the area of complex sample analysis by modern chromatographic and mass spectrometric methods. His research group (over 20 graduate and post-graduate scientists over the years at University of Patras and National and Kapodistrian University of Athens) focuses on the metabolic, quantitative analysis, pharmacokinetic and structure modification studies of drugs and bioactive compounds of pharmacological interest. He has established advanced bioanalytical methods that incorporate, ultra-performance liquid chromatographic techniques (UPLC-MS) and high-resolution tandem mass spectrometry techniques(UPLC-MS/MS).His group has been the pioneer in developing MS methodologies for detection of protein-ligand noncovalent complexes and determination of the ligand binding sites, and he has applied novel advancement into monitoring several biological systems such as that of the rasprotein and the β-amyloid peptide (Picture 1).

 

 

Research Interests and Goals

  • Studies of the metabolism and pharmacokinetics of pharmaceuticals and bioactive compoundsof pharmacological interest.
  • Detection and monitoring of noncovalent protein-ligand and protein-protein interactions under physiological conditions, which are responsible for certain diseases such as Alzheimer’s Disease (AD).
  • Provide insights in the protein aggregation processes involved in Alzheimer’s Disease (AD). Screening, evaluation and design of novel moleculespossessing anti-amyloidogenic activity.
  • Structure confirmation and post-translational studies of recombinant therapeutic proteins.

 

 

Research Infrastructure - Expertise

The existing infrastructure consists of Hewlett Packard gas chromatograph system (HP) 6890 GC coupled with HP 5973 mass spectrometer detector with single quadrupole (GC-MS) and HP 7683 autosampler. This system is used for pharmacokinetic studies of volatile substances (e.g., antidepressants, steroids). In the pharmacokinetic studies of antibiotics and other polar drugs ultra-high performance liquid chromatography (UPLC) coupled with a triple quadrupole (Micromass Waters TQD MS) or a quadrupole-time of flight mass spectrometer (Micromass Waters QTOF MS ) is used in collaboration with the Bioanalytical Laboratory of The Goulandris Natural History Museum (there is a collaboration agreement with the University of Athens Medical School - Pharmacology Laboratory).

 

 

Scientific Collaborations

The Pharmacokinetics & Protein-ligand Interactions group has developed national partnerships, such as those with the Pharmacognosy Labs of the Pharmacy Departments in University of Athens (Prof. A.L.Skaltsounis) and the University of Patras (Assoc. Prof. F. Lamari), the Bioanalytical Laboratory of the Goulandris Natural HistoryMuseum (Research program TreatAD) and the Neuropsychopharmacology group at the U. Athens Medical School (Ass. Prof. Ch. Dalla). It has also developed international collaborations with laboratories of Professor J. Bergquist, MD, PhD at UppsalaUniversity in Sweden, Prof. J. Griffin at University of Cambridge in England, and Prof. G. Allmaierat the Technical University of Vienna in Austria.

 

 

Organization of Scientific Conferences / Workshops

  • 2015 - Organization of the International Conference "The 3rd Mediterranean Sea Region Mass Spectrometry (MEDMS III) Workshop", Member of the International Organizing Committee, June 28 to 2 July 2015, Athens, Greece.
  • 2015 - Organization of the International "Workshop on Natural Products and Neurodegenerative Diseases", Member of the Organizing Committee, 25-27 June 2015, Athens, Greece.
  • 2010 - Organization of the International Conference "The 1st Mediterranean Sea Region Mass Spectrometry (MEDMS I) Workshop", Member of the International Organizing Committee of the Conference, 17-22 October 2010, Rehovot, Israel.
  • 2006 - Organization of the International Conference "DESORPTION 2006", President of the International and Local Organizing Committee of the Conference, 3-7 September 2006, Athens, Greece.

 

    

 

 

Selected European & Greek Research Programs

  • “Development and Screening of Novel beta Αmyloid Peptide Inhibitors for Alzheimer’s Disease (TreatAD)”. ESPA - National Action “Large Scale Cooperation Projects”(2012-2015).Coordinator of the Program.
  • “Structural elucidation of the bioactive components from the stigmas of Crocus Sativus L. by Tandem Mass Spectrometry.” Austrian Agency for International Cooperation in Education & Research, Academic Cooperation and Mobility Unit-ACM Grant. (2010).
  • “Structural study of bioactive components and mapping of the volatile components of Crocus Sativus L. by chromatographic and mass spectrometric methods.” Karatheodoris Grant – U. Patras (2007-2010).
  • “Evaluation of natural products towards the prevention and treatment of Alzheimer’s Disease”. Onassis Foundation Grant (2006-2008).Coordinator of the Program.
  • “Regulation of Soluble Guanylyl Cyclase: mapping the Phosphorylation Sites and determination of their biological importance.” PYTHAGORAS I (2004-2006).
  • Process development for an integrated olive oil mill waste management recovering natural antioxidants and producing organic fertilizer. LIFE 00 ENV/GR/000671 (2001-2004).
  • “Development of an economically viable process for the integrated management via utility of winemaking industry wastes; production of high added value natural products and organic fertilizer”. LIFE ENV/GR/000223 (2003-2006).
  • “Improving health through dietary phytoestrogens. A pan-European network on consumers’ issues and opportunities for producers”. PHYTOHEALTH/QLK1 CT 2002-02453 (2003-2006).
  • “Separation and quantitation of steroid hormones by gas chromatography mass spectrometry (GC/MS)-Application to the diagnosis of endocrine diseases, mainly of the hypothalamus-pituitary-adrenal gland axis”. PAVE GSRT (1999-2001).
  • “Environmental Estrogens and their Role in Human Health”. GSRT – Laboratory Networks (2000-2001).
  • “Analysis of the chemical composition and exploration of the role of bioactive compounds from Greek olive varieties and olive oil”.
    GSRT – PENED (2003-2005).
  • “Detection and determination of the bioactive componets of Greek wines and the corresponding varieties of vine trees (Vitisvinifera). Exploration of their role as chemo-preventive agents”. GSRT – PENED (2003-2005).
  • “Development of new biofunctional food and nutrition supplements based on plant ingredients of the Mediterranean diet and the plant biodiversity of the Greek flora”. GSRT – EPAN Nutrition (2003-2005).